Performance of HCV and HIV-1 Nucleic Acid Amplification Test(NAT) in Korean Blood Donors / 대한수혈학회지
Korean Journal of Blood Transfusion
;
: 91-97, 2000.
Artigo
em Coreano
| WPRIM
| ID: wpr-42776
ABSTRACT
BACKGROUND:
There is still risk of acquiring HCV and HIV by transfusion due to window phase. Screening for HCV and HIV-1 by nucleic acid amplification testing (NAT) may improve blood safety allowing detection during the preseroconversion window in donors.METHODS:
We investigated NAT usefulness using COBAS AMPLICOR analyzer (Roche). The following sample population were tested1) 15,552 HCV/HIV-1 seronegative random blood donor samples for HCV and HIV-1 NAT;2) 696 high ALT and 271 HCV EIA positive samples for HCV NAT;3) 1,152 HIV-1 EIA reactive samples for HIV-1 NAT. NAT was performed on pools of 24 donations according to the assay protocol. RESLUTS Six pools showed initial reactive reactions in HCV NAT and one pool showed initial reactive reaction in HIV-1 NAT. But no donor sample was found repeatedly reactive by this assay.CONCLUSIONS:
Although there were false positive reactions, specificity of the NAT assay was high enough for the assay to be applied as a blood screening test and implementation of this assay is expected to improve blood safety and be useful for blood products use.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Doadores de Tecidos
/
Doadores de Sangue
/
Programas de Rastreamento
/
Sensibilidade e Especificidade
/
HIV-1
/
HIV
/
Técnicas de Amplificação de Ácido Nucleico
/
Reações Falso-Positivas
/
Segurança do Sangue
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Blood Transfusion
Ano de publicação:
2000
Tipo de documento:
Artigo
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