Effects of all-trans retinoic acid, acitretin and tazarotene on apoptosis and active caspase-3 in human melanoma cells A375 / 中华医学美学美容杂志
Chinese Journal of Medical Aesthetics and Cosmetology
;
(6): 60-63, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-428359
ABSTRACT
Objective To investigate the effects of all -trans retinoic acid (ATRA),acitretin and tazarotene on apoptosis and active caspase-3 of human melanoma cells A375 and to discuss the relevant significance.Methods The effects of retinoids on apoptosis and active caspase-3 of A375 cells were examined in vitro.Early apoptosis analysis by double staining with annexin V-FITC and PI,and active caspase 3 analysis by the staining of FITC conjugated monoclonal rabbit anti active caspase-3 antibody were measured by flow cytometer.Results ATRA and acitretin induced apoptosis of A375 cells (both P<0.05),but tazarotene was not effective (P>0.05).ATRA and acitretin elevated the cell population with detectable active caspase-3 (both P<0.05),but tazarotene was not effective either (P>0.05).Acitretin played a most prominent role among the retinoids. Early apoptosis ratio was significantly and positively correlated with active caspase-3 percentage in both ATRA and acitretin (P<0.05).Conclusions Acitretin and ATRA induce apoptosis of A375 cells in which caspase is critical.Retinoids are prospectively applied as an assisting alternative for treating melanoma.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Medical Aesthetics and Cosmetology
Ano de publicação:
2012
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS