The effects of natural static magnetic fields on the proliferation of three human hepatocarcinoma cell lines / 中华物理医学与康复杂志

ABSTRACT

Objective To observe the effects of a natural static magnetic field (NSMF) on the proliferation of human hepatocarcinoma cells. Methods Three human hepatocarcinoma cell lines ( BEL-7402,HEP G-2 and QGY-7701 ) were cultured under standard conditions and then exposed to NSMFs of 0.1 Tesla at 600 Hz,0.2 Tesla at 250 Hz,0.2 Tesla at 400 Hz or 0.2 Tesla at 500 Hz for 30 minutes daily for 3 or 6 days.Flow cytometry and the XTT cell proliferation assay were used to check apoptosis and the proliferation of cells all groups. Results Only exposure to 0.2 Tesla magnetism at 400 Hz induced apoptosis of the BEL-7402 cell line.None of the NSMFs induced apoptosis in the other 2 hepatocarcinoma cell lines. Conclusions Different reactions of the three hepatocarcinoma cell lines to the same NSMF implies that each cell line has its own features,and that individualized NSMF treatment protocols should be adopted for better clinical outcomes.