Identification of a novel human leukocyte antigen allele, HLA-A * 31 ∶ 22 / 中华微生物学和免疫学杂志
Chinese Journal of Microbiology and Immunology
; (12): 1011-1014, 2012.
Article
em Zh
| WPRIM
| ID: wpr-429353
Biblioteca responsável:
WPRO
ABSTRACT
Objective To identify and confirm a novel HLA allele.Methods A new human leukocyte antigen A allele was found during routine HLA genotyping by polymerase chain reaction-sequence specific oligonucleotide probes(PCR-SSOP) and sequencing-based typing (SBT).HLA-A locus was amplified from exon 1 through exon 8,and the nucleotide sequence of exon 2 to exon 4 for HLA-A were sequenced in both directions.Results The novel HLA-A * 31 allele is identical to A * 31 ∶ 01 ∶ 02 with an exception of one base substitution at position 245 of exon 2 where an ' A' change to ' C' resulting in codon 82 changed from GAG to GCG.Conclusion A novel HLA allele,A * 31 ∶ 22,was identified,and was named officially by the WHO Nomenclature Committee for factors of the HLA system.
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1
Índice:
WPRIM
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
Zh
Revista:
Chinese Journal of Microbiology and Immunology
Ano de publicação:
2012
Tipo de documento:
Article