Study on molecular regulation mechanism of VEGF in HL-60 cells after induced differentiation / 国际肿瘤学杂志
Journal of International Oncology
;
(12): 873-877, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-429833
ABSTRACT
Objective To study the molecular regulation mechanism of VEGF in the model of ATRA induced differentiation in HL-60 cells,and to provide new targets for leukemia anti-angiogenic therapy.Methods The morphology was observed by Wright-Gimesa staining; HL-60 cells differentiation was detected by NBT reduction experiment.VEGF,STAT3,c-myc mRNAs were measured by reverse transcription-PCR;VEGF,STAT3 and c-myc proteins were determined by Western blot.Results The proliferation of HL-60 cells was inhibited obviously by ATRA(1 μmol/L) with the induction of differentiation,NBT positive rate was 82.59% (t =-24.157,P < 0.01) ; VEGF mRNA (t =7.339,P < 0.05),STAT3 mRNA (t =3.667,P <0.05) and c-myc mRNA (t =6.858,P < 0.05) were all down-regulated.VEGF protein (t =3.386,P <0.05),STAT3 protein(t =4.074,P < 0.05) and c-myc protein (t =3.333,P < 0.05) were all down-regulated.Conclusion VEGF expression level is reduced with the procession of differentiation of HL-60 cells,which may be largely correlated with the down regulation of STAT3 and c-myc.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo prognóstico
Idioma:
Chinês
Revista:
Journal of International Oncology
Ano de publicação:
2012
Tipo de documento:
Artigo
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