Effect of propofol on invasion of human liver cancer cell line HepG2 / 中华麻醉学杂志

ABSTRACT

Objective To investigate the effect of propofol on the invasion of human liver cancer cell line HepG2.Methods HepG2 cell were seeded in 96-well plates (100μl/hole) with a density of 1 × 105/ml and randomly divided into 5 groups (n =9 each)control group (group C),intralipid group (group Ⅰ),and propofol 30,60 and 120 μg/ml groups (groups P1-3).Propofol 30,60 and 120 μg/ml were added to the culture medium in groups P1-3,respectively,and then the cells were cultured for 48 h.In group Ⅰ,10％ intralipid was added to the culture medium and then the cells were cultured for 48 h.The invasion of cells was measured by Transwell invasion assay at 48 h of incubation.The expression of matrix metalloproteinases-2 (MMP-2) and mRNA and matrix metalloproteinases-9 (MMP-9) and mRNA was determined at 48 h of incubation.Results Compared with group C,the invasion of HepG2 cells was significantly decreased and the expression of MMP-2,MMP-9 and MMP-2 mRNA and MMP-9 mRNA was down-regulated in groups P1-3 (P ＜ 0.05),and no significant change was found in the parameters mentioned above in group Ⅰ (P ＞ 0.05).The invasion of HepG2 cells was gradually decreased and the expression of MMP-2,MMP-9 and MMP-2 mRNA and MMP-9 mRNA was gradually down-regulated in groups P1-3 (P ＜0.05).Conclusion Propofol can inhibit the invasion of HepG2 cells in a concentration-dependent manner and down-regulation of the expression of MMP-2 and MMP-9 may be involved in the mechanism.