Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis
Journal of Veterinary Science
;
: 491-494, 2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-43056
ABSTRACT
Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was used to evaluate clinical samples collected from naturally infected animals with D. congolensis. The results showed that this assay is a fast and reliable method for diagnosing dermatophilosis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Doenças dos Ovinos
/
Actinomycetales
/
Infecções por Actinomycetales
/
Ovinos
/
Doenças dos Bovinos
/
Reprodutibilidade dos Testes
/
Limite de Detecção
/
Reação em Cadeia da Polimerase em Tempo Real
/
Corantes Fluorescentes
/
Doenças dos Cavalos
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2013
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS