Photodynamic activity of novel targeting photosensitizer Ⅰ for Hep-2 cells / 肿瘤研究与临床
Cancer Research and Clinic
;
(6): 1-4, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-431486
ABSTRACT
Objective To evaluate the targeting and photodynamic activity of photosensitizer Ⅰ (PSⅠ)for laryngocarcinoma Hep-2 cells.Methods The photostability of PSⅠ was measured by bleaching assay.The cellular uptake of PSⅠ by Hep-2 cells were evaluated by fluorescence spectroscopy,the effects of PSⅠconcentration,irradiation dose and etc on the viability of Hep-2 cells were investigated by MTT assay.Results After irradiation of 50 min,the OD value of PS Ⅰ decreased 11%,which indicated that the stability of PSⅠ can meet the requirement of PDT.The cellular uptake of PS Ⅰ by Hep-2 cells increased with the concentration of PSⅠ and can be obviously inhibited by the presence of excessive free folic acid (P < 0.01).The results of MTT assays demonstrated that the viability of Hep-2 showed negative correlation with the PSⅠ concentration and irradiation dose.The viability of Hep-2 was only 34 % after PDT with 14 μ mol/L of the PS Ⅰ and 18 J/cm2 of irradiation.However,the viability of Hep-2 was still 100 % without irradiation,even though the concentration of PS Ⅰ was up to 1 10 μmol/L.The PDT carried out after 24 h of PSⅠ administration can efficiently inhibited the growth of Hep-2 with the survival rate of 32 %.Conclusion The PS Ⅰ posses satisfactory photostability and lower dark cytotoxicity,and displays significant targeting and photocytotoxicity for Hep-2 cells.
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Cancer Research and Clinic
Ano de publicação:
2013
Tipo de documento:
Artigo
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