The Effect of Antiproliferative Factor on the Proliferation of Bladder Cancer Cell and the Expression of c-Jun and HB-EGF / 中国医科大学学报

ABSTRACT

Objective To clarify the effect of antiproliferative factor（APF）on the proliferation of bladder cancer cell line T-24.Methods Active or no active APF with different concentrations was added to cultured bladder cancer T-24 cells.Methyl thiazolyl tetrazolium（MTT） assay was used to determine the proliferation of T-24 cells.The expression of c-Jun and HB-EGF were examined by Western blot.Results MTT showed that there was significant difference in the proliferation with the concentration of AFP increased from 0.5 μg /ml to 1.0 μg /ml.Compared with the negative control group,the absorption value of no active APF group showed no change（P 〉 0.05）.Western blot indicated that the c-Jun and HB-EGF expression were down-regulated with the concentration of active APF increased（P 〈 0.05）.No difference in their expression was found in no active APF groups.Conclusion Active APF may decrease the expression of c-Jun and HB-EGF,so as to inhibit the proliferation of bladder cancer cells.Our experiment might provide evidence for the therapeutic applications of APF for the bladder cancer.