The Promoting Role of bFGF on the Vascularization of Bone Matrix Gelatin Embedded Vascular Bundles in Rabbit Femoral Anteriomedialis / 中国医科大学学报

ABSTRACT

Objective To investigate the role of basic fibroblast growth factor（bFGF）on the vascularization of bone matrix gelatin（BMG） embedded vascular bundles in femoral anteriomedialis in the rabbits.Methods A longitudinal incision was done at the hyper-knee joint of femoral anteriomedialis in the Japan big ears white rabbit.Thirty six rabbits were divided into group A（n =12,arteria saphena and vena saphena were liberated and embeded to the groove of BMG soaked by 2 μg/ml bFGF0,group B（n =12,arteria saphena and vena saphena were liberated and embeded to the groove of BMG untreated）,and group C（n =12,the BMG untreated were directly implanted）.The rabbits were sacrificed at 4 weeks,8 weeks and 12 weeks after the surgery respectively by perfusing ink.After the implants were dislodged,transparent specimens were made and Masson stained for histological observations and quantitative analysis.Results After 12 weeks of operation,the neovascularization arranged in an ordered manner in group A,gradually trended to be orderly in group B,and were cluttered mainly on the edge of the implants in group C.The osteogenic and neovascularization areas of group A were the largest on each time point.Conclusion bFGF could promote the vascularization of BMG embedded vascular bundle.There was a positive correlation between osteogenesis and vascularization.