Effects of selective cyclooxygenase-2 inhibitor NS-398 on apoptosis and survivin, XIAP and c-IAP1 expressions of hepatocellular carcinoma BEL-7402 cells / 肿瘤
Tumor
; (12): 11-14, 2010.
Article
em Zh
| WPRIM
| ID: wpr-433066
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WPRO
ABSTRACT
Objective:To investigate the effect and elucidate the mechanism of the selective cyclooxygenase-2(COX-2)inhibitor NS-398 on apoptosis and survivin, XIAP and c-IAP1 expressions of hepatocarcinoma cell lines. Methods:The proliferation of hepatocarcinoma BEL-7402 cells treated with NS-298 at different concentrations were evaluated by MTT assay. The apoptosis was detected by flow cytometry (FCM) and TUNEL assay. Expressions of COX-2, survivin, XIAP and c-IAP1 were analyzed by immunocytochemical staining. Results: NS-398 significantly inhibited cell proliferation of BEL-7402 cells and induced apoptosis. Immunocytochemisty indicated that the expressions of COX-2, survivin, XIAP and c-IAP1 were significantly down-regulated in BEL-7402 cells by NS-398 treatment compared with untreatment group (P<0.01). Conclusion:NS-398 inhibits the proliferation and induced apoptosis of BEL-7402 cells. The mechanism may be related with down-regulation of the survivin, XIAP and c-IAP1 expression.
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WPRIM
Idioma:
Zh
Revista:
Tumor
Ano de publicação:
2010
Tipo de documento:
Article