Simultaneous determination of two compounds in Mosla chinensis by RP-HPLC / 中成药
Chinese Traditional Patent Medicine
;
(12): 88-91, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-433190
ABSTRACT
AIM:
To establish a method for simultaneously determining luteolin and negletein in Mosla chinensis Maxium collected in picking times.METHODS:
The Angilent Zorbax,Extend C-18 column(4.6 mm×250 mm,5μm) was used with methanol-water (containing 0.4% phosphonic acid)(50 50) as mobile phase.The flow rate was 1.0 mL/min.The detection wavelength was set at 350 nm.RESULTS:
The method had the good linear relationship within the range of 0.04-0.61 μg for luteolin and within the range of 0.20-3.01 μg for negletein,respectively,with the correlation coefficient of 0.999 9.The average recoveries of the two flavones were 97.32%,98.60%,respectively.The contents of luteolin and negletein in Mosla chinensis Maxium.varied in different picking times,which reached the its maximum at florescence (264.8 μg/g,2 746.3 μg/g) in August,respectively.CONCLUSION:
The method is fast,rapid,convenient,accurate and can be used for quality control of Mosla chinensis Maxium.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Traditional Patent Medicine
Ano de publicação:
2010
Tipo de documento:
Artigo
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