Antimicrobial resistance and BRO genotyping of Moraxella catarrhalis isolates from respiratory tract in children / 临床儿科杂志
Journal of Clinical Pediatrics
;
(12): 719-722, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-433417
ABSTRACT
Objectives To investigate antimicrobial resistance and beta-lactamase production of Moraxella catarrhalis isolates from respiratory tract in children and to understand the characteristics of BRO beta-lactamase gene. Methods From June 2011 to Sep-tember 2012, 401 Moraxella catarrhalis isolates were obtained from respiratory tract in children. Minimum inhibitory concentrations (MIC) of commonly-used antibiotics were determined by microbroth dilution assay, and beta-lactamase production was detected by Nitroceifn disk test. PCR combining restriction endonuclease analysis was employed to do the BRO genotyping. Results 96.5%iso-lates were beta-lactamase positive (387/401), MIC (MIC50/MIC90) values and resistant rates of beta-lactamase producing isolates were higher than those of non beta-lactamase producing isolates for ampicillin, cefaclor and cefuroxime (P<0.05). The positive rate of BRO gene was 99.2%in beta-lactamase producing isolates (384/387), consisting of 93.0%BRO-1 isolates and 7.0%BRO-2 isolates. MIC50 and MIC90 values of BRO-1+isolates were higher than those of BRO-2+isolates for ampicillin, cefaclor, cefuroxime and azithromycin. Conclusions The beta-lactamase production rate is high in Moraxella catarrhalis isolates from respiratory tract in children. BRO-1 type was the dominant genotype of beta-lactamase producing isolates, having more inlfuence than BRO-2 type in the inlfuence on some beta-lactams and macrolides.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Clinical Pediatrics
Ano de publicação:
2013
Tipo de documento:
Artigo
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