HPLC Determination of Saponins in Zhixiao Suppositories / 中国实验方剂学杂志
Chinese Journal of Experimental Traditional Medical Formulae
;
(24): 25-27, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-434167
ABSTRACT
Objective:
To denvolop HPLC an method for the determination of the content of three saponins in Zhixiao suppositories.Methods:
Analysis carriedout was performed on a Kromasil C18 column (4.6 mm×250 mm., 5μm)at 23℃. The mobile phases were methanol and 0.2% phosphoricacid solution in a gradient elution. The flow rate was set at 1.0 mL·min-1 and the ultraviolet detector was operated at 203 nm.Results:
The linear ranges of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 were 0.368~2.76μg,0.676~5.07 μg and 0.812~6.09μg,respectively. The average recoveries of the three saponins were 98.53%, 99.20% and 99.35%, respectively. The RSD (n=5) were 2.11%, 1.88% and 2.38%, respectively.Conclusion:
The separation is a good and the results are accurate and reproducible. It can be used for the quality control of Zhixiao suppositories.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Experimental Traditional Medical Formulae
Ano de publicação:
2009
Tipo de documento:
Artigo
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