Elaboration of protocol in dosage of ferulic acid in Radix angelicae sinensis by HPLC

ABSTRACT

The extracted and purified ferulic acid residue from Radix angelicae sinensis was quantified by an established HPLC system composed of the following conditions reversed phase column C-18 Purosphere 4.6mm 1D x 250 mm (5mm); mobile phase acetonitrile/potassium dihydrophosphate 10 mM/L (15/85); pH 4.5; flow rate 1.2 mL/min; column temperature 30oC. The results showed that the method can meet the following criteria Rs > 1.5, RSD 1 000, Tf in the range 0.8-1.2