Epitope competition weakens specific T cell response to subdominant epitopes / 中华微生物学和免疫学杂志
Chinese Journal of Microbiology and Immunology
; (12): 440-444, 2013.
Article
em Zh
| WPRIM
| ID: wpr-436516
Biblioteca responsável:
WPRO
ABSTRACT
Objective To elucidate the influences of epitope competition on the frequency and average intensity of specific T cell response.Methods C57BL/6 mice were immunized with either single epitope DNA vaccines (pSV-gag92 or pSV-env203) or fusion gene DNA vaccine (pSV-gag/env).Gag92and Env203 epitope-specific CD8 T cell responses were analyzed by intracellular cytokine staining assay.Results Gag92-specific IFN-γ+CD8 T cells that were induced by pSV-gag92 accounted for 0.415 00% ±0.045 88% of the total CD8 T cells,which was much more than that induced by pSV-gag/env of 0.058 67% + 0.019 64%.Moreover,the mean fluorescence intensity of Gag92-specific TNF-α-IFN-γ+CD8 T cells (296.70+14.08) elicited by pSV-gag/env was significantly lower than that of Env203-specific TNF-α-IFN-γ+CD8 T cells (818.00+49.34).Conclusion Epitope competition could significantly decrease both the frequency and the average intensity of specific T cell response to subdominant epitopes.
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WPRIM
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Zh
Revista:
Chinese Journal of Microbiology and Immunology
Ano de publicação:
2013
Tipo de documento:
Article