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Apoptosis and upregulation of TNF-alpha and TRAIL receptor 1 (DR4) in the pathogenesis of food protein-induced enterocolitis syndrome / 소아과
Korean Journal of Pediatrics ; : 525-531, 2010.
Artigo em Coreano | WPRIM | ID: wpr-43756
ABSTRACT

PURPOSE:

Expression levels of tumor necrosis factor (TNF)-alpha expression on the mucosa of the small intestine is increased in patients with villous atrophy in food protein-induced enterocolitis syndrome (FPIES). TNF-alpha has been reported to induce apoptotic cell death in the epithelial cells. We studied the TNF family and TNF-receptor family apoptosis on the duodenal mucosa to investigate their roles in the pathogenesis of FPIES.

METHODS:

Fifteen infants diagnosed as having FPIES using standard oral challenge test and 5 controls were included. Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining was performed to identify the apoptotic cell death bodies. Immunohistochemical staining of TNF-alpha, Fas ligand (FasL) for TNF family and TNF-related apoptosis-including ligand (TRAIL) receptor 1 (DR4), TRAIL receptor 2 (DR5), and Fas for TNF-receptor family were performed to determine the apoptotic mechanisms.

RESULTS:

TUNEL+ was significantly more highly expressed in the duodenal mucosa of FPIES patients than in controls (P=0.043). TNF-alpha (P=0.0001) and DR4 (P=0.003) were significantly more highly expressed in FPIES patients than in controls. Expression levels of FasL, Fas, and DR5 were low in both groups and were not significantly different between the 2 groups.

CONCLUSION:

These results suggest that FPIES pathogenesis is induced by apoptosis, and that TNF-alpha expression and DR4 pathway may have an important role in apoptosis.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Atrofia / Regulação para Cima / Fator de Necrose Tumoral alfa / Morte Celular / Apoptose / Enterocolite / Células Epiteliais / Proteína Ligante Fas / Receptores do Ligante Indutor de Apoptose Relacionado a TNF / Intestino Delgado Tipo de estudo: Estudo de etiologia / Estudo prognóstico Limite: Humanos / Lactente Idioma: Coreano Revista: Korean Journal of Pediatrics Ano de publicação: 2010 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Atrofia / Regulação para Cima / Fator de Necrose Tumoral alfa / Morte Celular / Apoptose / Enterocolite / Células Epiteliais / Proteína Ligante Fas / Receptores do Ligante Indutor de Apoptose Relacionado a TNF / Intestino Delgado Tipo de estudo: Estudo de etiologia / Estudo prognóstico Limite: Humanos / Lactente Idioma: Coreano Revista: Korean Journal of Pediatrics Ano de publicação: 2010 Tipo de documento: Artigo