Screening Effective Components from Fructus Citri Sarcodactylis by Cell Membrane Chromatography and HPLC/MS / 中国中医药信息杂志
Chinese Journal of Information on Traditional Chinese Medicine
;
(12): 66-68, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-438332
ABSTRACT
Objective To screen the active component from Fructus Citri Sarcodactylis which have aortic vascular relaxant effect. Methods The active components from Fructus Citri Sarcodactylis were screened by combining vascular smooth muscle/cell membrane chromatography (VSM/CMC) and liquid chromatography/mass spectrometry (LC/MS) with pharmacological assay in vitro. Results Bergapten was the active component of Fructus Citri Sarcodactylis that functions in vascular. The pharmacological experiment showed that bergapten had vasodilating effect. The capacity factor of this compound on the VSM/CMC model was found to be significantly correlated with its pharmacological effect. Conclusion Association between compound and membrane protein (receptor) can be reflected by using the VSM/CMC model. VSM/CMC-offline-LC/MS technology can be used to quickly screening new active components from natural medicine.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
/
Estudo de rastreamento
Idioma:
Chinês
Revista:
Chinese Journal of Information on Traditional Chinese Medicine
Ano de publicação:
2013
Tipo de documento:
Artigo
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