Relationship of IL-8/-251 gene polymorphisms with incidence of sepsis in patients with severe traumatic injury / 中华创伤杂志
Chinese Journal of Trauma
;
(12): 1220-1223, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-439212
ABSTRACT
Objective To assess the clinical relevance of polymorphisms at position-251 in the promoter region of IL-8 gene and the incidence of sepsis in patients with severe trauma.Methods A total of 296 patients with severe trauma were included in the prospective cohort study.Incidence of sepsis was decided on the basis of the clinical manifestations and blood culture results.Muhiple organ dysfunction score (MODS) was performed using Marshall' s standard.IL-8/-251 gene polymorphisms were genotyped using restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP).IL-8 plasma level was determined using ELISA method.Results Genotype frequency at IL-8/-251 locus in trauma patients was in accord with Hardy-Weinberg equilibrium (P > 0.05).Sepsis incidence in trauma patients with TT,TA,and AA genotypes at IL-8/-251 locus was 58.1%,49.6%,and 25.0% respectively.Multiple regression analysis showed inverse correlation between sepsis incidence and quantity of A alleles [OR =0.637,95% CI (0.421,0.963),P < 0.05].Carriers of AA genotype presented lower MODS score than those of other two genotypes (P < 0.05).IL-8 plasma level presented significant difference among carriers of the three genotypes (P < 0.01) and A alleles were associated with the down-regulation of IL-8 (P < 0.01).Conclusion IL-8/-251T/A polymorphisms are implicated in the development of posttraumatic sepsis and AA genotype is protective against posttraumatic sepsis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo de incidência
/
Estudo observacional
Idioma:
Chinês
Revista:
Chinese Journal of Trauma
Ano de publicação:
2013
Tipo de documento:
Artigo
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