Intracellular Localization of the Porcine Reproductive and Respiratory Syndrome Virus Nucleocapsid Protein
Journal of Bacteriology and Virology
;
: 29-37, 2008.
Artigo
em Coreano
| WPRIM
| ID: wpr-43954
ABSTRACT
Porcine reproductive and respiratory syndrome virus (PRRSV) is a small enveloped, positive-stranded RNA virus belonging to the family Arteriviridae. It causes the porcine reproductive and respiratory syndrome in swine. The virus has 7 structural proteins Of the seven, the N protein is the nucleocapsid that comprises a core of the virus particle. We have expressed the N protein of PRRSV PL97-1/LP1 strain using a heterologous gene expression vector derived from Sindbis virus, called pSinrep5. Immunofluorescence analysis showed that the N proteins were mainly found in the cytoplasm as well as in the nucleus of BHK-21 cells transfected with pSinrep5-N-derived RNA. Moreover, expression of the N protein did not change the incompetence of RNA replication of Mutant/nt14900 that lacks a 3' cis-acting replication element and the efficiency of RNA replication of Mutant/nt14800 that has a low level of RNA replication. Overall, our findings are consistent with previous results and help to understand a role of the N protein in PRRSV biology.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Vírus de RNA
/
Sindbis virus
/
Entorses e Distensões
/
Suínos
/
Vírion
/
Vírus
/
RNA
/
Proteínas
/
Expressão Gênica
/
Imunofluorescência
Limite:
Humanos
Idioma:
Coreano
Revista:
Journal of Bacteriology and Virology
Ano de publicação:
2008
Tipo de documento:
Artigo
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