Compatibility of human osteoblasts and composite porous bioscaffold / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 8869-8874, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-440405
ABSTRACT
BACKGROUND:
The degradation of a col agen Ⅰ modified porous calcium sulfate scaffold in vivo is unclear, and its degradation product effects on human osteoblasts are rarely reported.OBJECTIVE:
To observe the biocompatibility of human osteoblasts with degradation products of calcium sulfate/col agen membrane composite porous scaffold.METHODS:
Passage 2 human osteoblasts were cultured in the extract of degradation products of calcium sulfate/col agen membrane composite porous scaffold and in Dulbecco’s modified Eagle’s medium containing 10%newborn calf serum. At days 1, 3, 5, 7, cellproliferative curves and total protein were determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method and Coomassie bril iant blue micro-plate method, respectively. And alkaline phosphatase activity was also detected. RESULTS ANDCONCLUSION:
The proliferation rate of human osteoblasts in the extract of degradation products of calcium sulfate/col agen membrane composite porous scaffold was slightly higher than that in the Dulbecco’s modified Eagle’s medium containing 10%newborn calf serum, but there was no significant difference (P>0.05). Alkaline phosphatase activity, total protein synthesis and alkaline phosphatase/total protein were increased with time in the two groups, but there was no significant difference at different time (P>0.05). These findings indicate that the degradation products of calcium sulfate/col agen membrane composite porous scaffold cannot influence proliferation and growth of human osteoblasts as wel as their normal physiological functions, which have good biocompatibility.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Tissue Engineering Research
Ano de publicação:
2013
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS