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Palmitate induces cell apoptosis in insulinoma MIN6 cell / 基础医学与临床
Basic & Clinical Medicine ; (12): 401-405, 2010.
Article em Zh | WPRIM | ID: wpr-440640
Biblioteca responsável: WPRO
ABSTRACT
Objective To investigate the effect of protein kinase B and its phosphorylation on the apoptosis of MIN6 induced by palmitate.Methods Incubated with different level of palmitate concentration (0 ~ 0.5 mmol/L),MIN6 cells were cultured in high glucose DMEM with or without LY294002 ; the apoptosis of MIN6 cells was detec-ted and quantified with TUNEL technology.Then we inspected the cell ultrastructure through electron microscopy and determined the expression of protein kinase B and its phosphorylation p-PKB (Ser473) by Western blot,fol-lowed by an RT-PCR detection of BAX and BCL-2 expression.Results Apoptosis of MIN6 cells was induced by palmitate in a concentration-dependent correlation and enhanced by LY294002.Palmitate also inhibited phospho-rylation of protein kinase B on Ser473.Finally,we detected a downregulation of BCL-2 mRNA expression and up-regulation of BAX mRNA expression under palmitate-incubated state.Conclusion Palmitate may induce apoptosis of pancreatic β-cells through inhibiting activation of PKB phosphorylation in diabetes.
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Texto completo: 1 Índice: WPRIM Idioma: Zh Revista: Basic & Clinical Medicine Ano de publicação: 2010 Tipo de documento: Article
Texto completo: 1 Índice: WPRIM Idioma: Zh Revista: Basic & Clinical Medicine Ano de publicação: 2010 Tipo de documento: Article