Microvessel density and neovascularization of endometrium after uterine artery embolization with trisacryl gelatin microspheres / 中国组织工程研究

ABSTRACT

BACKGROUND:Uterine arterial embolization is a minimal y invasive technique and widely used for the treatment of several obstetrics and genecology diseases. However, reports about the influence of uterine arterial embolization on the pathophysiological microvessel density and angiogenesis changes in the endometrium have been very limited. OBJECTIVE:To investigate the influence of uterine artery embolization on endometrial microvessel density and neovascularization. METHODS:Sixty female guinea pigs were randomly divided into control group (n=15) and uterine artery embolization group (n=45). In the uterine artery embolization group, trisacryl gelatin microspheres (Embosphere) was used to perform uterine artery embolization and animals were further divided into three subgroups including E1, E2, and E3 (n=15 in each subgroup). Uterine specimens were col ected at 7-15 days, 16-30 days, and 31-45 days after uterine artery embolization respectively in the three subgroups. RESULTS AND CONCLUSION:Microspheres were visible in the lumen of primary branch of uterine artery, subserosal arteriole and intramuscular arteriole. Statistical analysis demonstrated that CD34-positive microvessel density of the endometrial basal layer significantly decreased after uterine artery embolization, and reverted to the normal level (P<0.05). Increase of neovascularization with CD105-postive microvessel density presented after uterine artery embolization, and then returned to the normal (P<0.05). These findings indicate that uterine artery embolization may lead to a temporal decrease of microvessel density in the endometrium which wil recover over time as a result of the increase of CD105-postive neovascularization.