Role of rVvhA in inducing THP-1 cells damage / 中华微生物学和免疫学杂志
Chinese Journal of Microbiology and Immunology
; (12): 761-765, 2013.
Article
em Zh
| WPRIM
| ID: wpr-441806
Biblioteca responsável:
WPRO
ABSTRACT
Objective To investigate the role of recombinant Vibrio vulnificus cytolysin (rVvhA) in inducing THP-1 cells damage and study the pathway of associated calcium influx .Methods Inverted mi-croscope, CCK-8 cell proliferation kit, Fluo3/AM staining and caspase activity detection were performed to analyze the damage of THP-1 cells induced by rVvhA and the pathway of calcium influx .Results rVvhA had cytotoxic effects on THP-1 cells in a dose-dependent manner .The concentrations of extracellular K +and LDH were respectively up-regulated after 1 h and 6 h of 12 μg/ml rVvhA intervention .Verapamil , Mibe-fradil and SKF-96365 could not prevent the influx of free Ca 2+induced by rVvhA .The activities of caspase-3 and caspase-9 were singanificantly enhanced by rVvhA in a time-dependant manner .Conclusion rVvhA can induce THP-1 cells damage through triggering extracellular calcium influx via porous channel on cell membrane.Moreover, rVvhA might induce THP-1 cell apoptosis through activating caspase-9/3-dependent pathway .
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WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Microbiology and Immunology
Ano de publicação:
2013
Tipo de documento:
Article