Comparison of the Chemical Composition of Subchondral Trabecular Bone of Medial Femoral Condyle between with Advanced Osteoarthritis and without Osteoarthritis
Journal of Bone Metabolism
;
: 93-97, 2015.
Artigo
em Inglês
| WPRIM
| ID: wpr-44195
ABSTRACT
BACKGROUND:
The purpose of this study is to investigate differences of chemical composition between subchondral bone in advanced osteoarthritic (OA) and non-OA distal femur.METHODS:
Twenty femurs were harvested, respectively. The subchondral trabeculae were obtained from the middle of medial articular surface of distal femurs. A 10 mm diameter cylindrical saw was used to harvest. Raman spectroscopy, a non-destructive technique, was employed to determine the chemical information of the trabecular bones in the human distal femurs.RESULTS:
The maximum intensity of the phosphate peak was 2,376.51+/-954.6 for the non-OA group and 1,936.3+/-831.75 for the OA group. The maximum intensity of the phosphate peak observed between the two groups was significantly different (P=0.017). The maximum intensity of the amide I peak were 474.17+/-253.42 for the nonOA group and 261.91+/-205.61 for the OA group. The maximum intensity of the amide I peak were significantly different between the two groups (P=0.042). Also, among other chemical and matrix components (Hydroxyproline,Carbonate, Amide IIIdisordered;ordered, and CH2), the spectrums showed similar significant differences in the intensity (P=0.027, P=0.014, P=0.012; P=0.038, P=0.029). Area integration were performed to determine disorder in collagen's secondary structure via amide III (alpha helix/random coil). The value of the alpha helix to random coil band area are significantly different (P=0.021) and result showing that there was a trend toward higher collagen maturity for the nonosteoarthritic bone specimens.CONCLUSIONS:
The result suggested that OA may affect the chemical compositions of trabecular bone, and such distinctive chemical information may be.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoartrite
/
Análise Espectral Raman
/
Cartilagem
/
Colágeno
/
Fêmur
Limite:
Humanos
Idioma:
Inglês
Revista:
Journal of Bone Metabolism
Ano de publicação:
2015
Tipo de documento:
Artigo
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