Expression and significance of Wnt1 and β-catenin proteins in breast invasive ductal carcinoma / 中国医师杂志

ABSTRACT

Objective To investigate the expressions of Wnt1 and β-catenin proteins in mammary cancer and its relationship with clinical pathological characteristics,and explore its role in the pathogenesis of breast cancer.Methods Immunoshistochemisty (SP method) was used to detect the expressions of Wnt1 and β-catenin proteins in adjacent tissue of breast cancer (n =15),breast hyperplasia tissues (n =25),and breast in vasive ductal carcinoma (n =80).SPSS 17.0 statistical software was used for analysis,such as count data with x2 test and correlation analysis with Spearman test,with significance level of α =0.05.Results The positive rate of Wnt1 and β-catenin in breast cancer group [62.5％ (50/80) and 68.8 (55/80)] was significantly higher than that in hyperplasia group [28％ (7/25) and 20％ (5/25)]and in borderline group [13.33 (2/15) and 13.33 (2/15)] with a significant difference (P ＜ 0.01).Positive expression of Wnt1 and abnormal expression of β-catenin in breast invasive ductal carcinoma were related to lymphatic metastasis (x2 =5.10,4.87,P ＜ 0.05).Abnormal expression of β-catenin was also related to the histological grading of breast invasive ductal carcinoma (x2 =5.61,P ＜ 0.05).The expressions of Wnt1 and β-catenin proteins in invasive ductal carcinoma showed a positive correlation (r =0.313,P ＜ 0.01).Conclusions The high level expression of Wnt1 protein and the abnormal expression of β-catenin protein might play an important role in breast carcinogenesis,and was related to lymph node metastasis.