Bone marrow mesenchymal stem cells repair vascular injury after cervical spondylotic vertebral arteriopathy / 中国组织工程研究

ABSTRACT

BACKGROUND:Inflammation is an important factor in cervical spondylotic vertebral arteriopathy, bone marrow mesenchymal stem cells have the potential to treat cervical spondylotic vertebral arteriopathy because of the immunomodulatory effects to inhibit inflammation. OBJECTIVE:To investigate the injury mechanism of vascular injury in the model of cervical spondylotic vertebral arteriopathy and to study the therapeutic effects of bone marrow mesenchymal stem cells on it. METHODS:Forty Japanese big ear rabbits were randomly divided into four groupscontrol group, model group, tanshinone group, and stem cellgroup. After modeling, the control and model groups were not given intervention, while the tanshinone and stem cellgroups were injected with tanshinone II A sodium sulfonate solution (10 mL) and bone marrow mesenchymal stem cellsuspension (10 mL) along the ear vein, respectively. After 2 weeks, the routine pathological examination was done to observe the vascular morphological changes, immunofluorescence staining was done to observe the cathepsin B expression in the vertebral artery, and ELISA was used to detect the expression of tumor necrosis factor-αand interleukin-6 in the vertebral artery. RESULTS AND CONCLUSION:Compared with the model group, the arterial smooth muscle cellhypertrophy and hyperplasia was obviously restrained in stem cellgroup, and vascular endothelial fold was in symmetry, while no significant difference was found between stem cellgroup and tanshinone group. Compared with the model group, expressions of cathepsin B, interleukin 6 and tumor necrosis factor-αexpression were reduced significantly in the stem cellgroup (P0.05). Inflammatory reaction may be one of mechanisms for vertebral artery damage, and bone marrow mesenchymal stem cells can effectively inhibit inflammation of the vertebral artery and improve vascular remodeling.