Identification of Tumor Suppressor Genes on Chromosome 21 / 대한흉부외과학회지
The Korean Journal of Thoracic and Cardiovascular Surgery
;
: 141-147, 2009.
Artigo
em Coreano
| WPRIM
| ID: wpr-44393
ABSTRACT
BACKGROUND:
We performed this study to identify the tumor suppressor genes located in the long arm of chromosome 21 in non-small cell lung cancer. MATERIAL ANDMETHOD:
The genes of USP25 in 21q11.2, NCAM2, ADAMTS1 in 21q21.2, and Claudin-8 (CLDN8), Claudin-17 (CLDN17) and TIAM1 in 21q22.1 were investigated for their gene expressions, genetic alterations and promoter methylation.RESULT:
The expressions of CLDN8 and CLDN17 were significantly decreased in 7 (L132, H157, H358, H522, H1299, H1703 and HCC2108) of 13 cell lines, and the expression of ADAMTS1 was also significantly reduced in 6 cell lines (A549, SW900, H1299, H1373, H1703 and H1793). There were no genetic alterations by PCR-SSCP and cDNA cloning in the cell lines with a decreased gene. In the cell lines with a decreased gene expression, the mRNA expression was increased significantly with treatment of 5-Aza-CdR.CONCLUSION:
These results suggest that the ADMTS1, CLDN8 and CLDN17 may act as tumor suppressor genes.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Braço
/
Cromossomos Humanos Par 21
/
RNA Mensageiro
/
Expressão Gênica
/
Linhagem Celular
/
Genes Supressores de Tumor
/
Células Clonais
/
DNA Complementar
/
Carcinoma Pulmonar de Células não Pequenas
/
Clonagem de Organismos
Tipo de estudo:
Estudo diagnóstico
Idioma:
Coreano
Revista:
The Korean Journal of Thoracic and Cardiovascular Surgery
Ano de publicação:
2009
Tipo de documento:
Artigo
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