Vascularization of poly(hydroxybutyrate-co-hydroxyoctanoate) osteochondral scaffold in vitro / 中国组织工程研究

ABSTRACT

BACKGROUND:The poly(hydroxybutyrate-co-hydroxyoctanoate) osteochondral scaffold which has been constructed in previous experiments has good biocompatibility and biodegradability and generates non-toxic degradation products. OBJECTIVE:To observe the vascularization of rabbit renal microvascular endothelial cels co-cultured with poly(hydroxybutyrate-co-hydroxyoctanoate) osteochondral scaffold. METHODS:The poly(hydroxybutyrate-co-hydroxyoctanoate) osteochondral scaffold having a three-layer structure (layer of bone/bone and cartilage interface layer/layer of cartilage) was prepared by solvent casting/particle leaching method. The renal microvascular endothelial cels at passage 3 were seeded onto the scaffold of bone layer. The proliferation of the renal microvascular endothelial cels growing on the scaffolds was examined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method, the growth of cels in the scaffold was observed by hematoxylin-eosin staining under electron microscope after 10 days. RESULTS AND CONCLUSION:The integrated osteochondral scaffold had a clear appearance of three-layer structure, which had closed connections between the three layers. Porous bone layer was visible as wel as uniform and interlinked pores, and the porosity was 78%. The renal microvascular endothelial cels seeded onto the scaffold proliferated wel and presented a three-dimensional growth after 10 days of co-culture, but there were no cels on the interface layer. Cels which adhered and grew between the pores of the bone layer were observed through hematoxylin-eosin staining. Cels showed a luminal-like structure growing on the scaffold with the porous structure, but they did not grow into the interface layer of bone and cartilage.