Establishment of a Protocol for Determining Gastrointestinal Transit Time in Mice Using Barium and Radiopaque Markers
Korean Journal of Radiology
;
: 45-50, 2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-44596
ABSTRACT
OBJECTIVE:
The purpose of this study was to establish a minimally invasive and reproducible protocol for estimating the gastrointestinal (GI) transit time in mice using barium and radiopaque markers. MATERIALS ANDMETHODS:
Twenty 5- to 6-week-old Balb/C female mice weighing 19-21 g were used. The animals were divided into three groups two groups that received loperamide and a control group. The control group (n = 10) animals were administered physiological saline (1.5 mL/kg) orally. The loperamide group I (n = 10) and group II (n = 10) animals were administered 5 mg/kg and 10 mg/kg loperamide orally, respectively. Thirty minutes after receiving the saline or loperamide, the mice was administered 80 microL of barium solution and six iron balls (0.5 mm) via the mouth and the upper esophagus by gavage, respectively. Afterwards, the mice were continuously monitored with fluoroscopic imaging in order to evaluate the swallowing of the barium solution and markers. Serial fluoroscopic images were obtained at 5- or 10-min intervals until all markers had been excreted from the anal canal. For analysis, the GI transit times were subdivided into intestinal transit times (ITTs) and colon transit times (CTTs).RESULTS:
The mean ITT was significantly longer in the loperamide groups than in the control group (p < 0.05). The mean ITT in loperamide group II (174.5 +/- 32.3) was significantly longer than in loperamide group I (133.2 +/- 24.2 minute) (p < 0.05). The mean CTT was significantly longer in loperamide group II than in the control group (p < 0.05). Also, no animal succumbed to death after the experimental procedure.CONCLUSION:
The protocol for our study using radiopaque markers and barium is reproducible and minimally invasive in determining the GI transit time of the mouse model.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Próteses e Implantes
/
Propriedades de Superfície
/
Sulfato de Bário
/
Trânsito Gastrointestinal
/
Fluoroscopia
/
Microscopia Eletrônica de Varredura
/
Cloreto de Sódio
/
Reprodutibilidade dos Testes
/
Análise de Variância
/
Meios de Contraste
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Korean Journal of Radiology
Ano de publicação:
2013
Tipo de documento:
Artigo
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