Expression and function of calcium-sensing receptor in mouse embryonic stem cells / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12): 651-657, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-446063
ABSTRACT
AIM:
To observe the functional expression of calcium-sensing receptor (CaSR) in the mouse em-bryonic stem cells (mESCs).METHODS:
The expression and distribution of CaSR were detected by Western blotting and immunofluorescence observation in 129 mouse ES-D3 cells.The intracellular concentration of free calcium ([Ca2+]i) was determined by confocal laser scanning microscopy .The cell viability was analyzed by MTT assay and flow cytometry .RE-SULTSCaSR protein was expressed in mESCs .Extracellular calcium or neomycin significantly increased the expression of CaSR and [Ca2+]i.Neomycin increased the cell viability , up-regulated the protein expression of p-ERK2.These effects of neomycin were inhibited by NPS2390.CONCLUSION:
CaSR is expressed in mESCs .The activation of CaSR is involved in the proliferation of mESCs .
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Pathophysiology
Ano de publicação:
2014
Tipo de documento:
Artigo
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