Pro-Apoptosis Effects of Danhong Injection on the SMMC-7721 Hepatoma Cells / 医药导报
Herald of Medicine
; (12): 565-568, 2014.
Article
em Zh
| WPRIM
| ID: wpr-446267
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WPRO
ABSTRACT
Objective To investigate the effects of danhong injection on the apoptosis of SMMC-7721 heptoma cells.Methods The hepatocellular carcinoma cell line SMMC-7721 was incubated with different concentrations(0,2,4,8 μL·mL-1) of danhong injection, and cell morphology was studied under the microscope. The apoptosis index was detected by flow cytometry at 0,6,12,24 h after cultured with 2 μL·mL-1danhong injection. Cell proliferation was measured by MTT assay;Gene expressions of p53,Bax and Bcl-2 were detected by RT-PCR at different drug concentrations. Results The morphology of the hepatoma cells changed obviously,and the apoptosis index increased by danhong injection in a dose-dependant manner. The danhong injection decreased gene expressions of Bcl-2,and obviously increased p53 and Bax. Conclusion Danhong injection can dose-and time-dependently promote apoptosis of SMMC-7721 cells.
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WPRIM
Idioma:
Zh
Revista:
Herald of Medicine
Ano de publicação:
2014
Tipo de documento:
Article