Plasma G-CSF and GM-CSF Concentrations and Expression of their Receptors on the Granulocyte in Children with Leukocytosis
Journal of the Korean Pediatric Society
;
: 271-276, 2003.
Artigo
em Coreano
| WPRIM
| ID: wpr-44750
ABSTRACT
PURPOSE:
Granulocyte-colony stimulating factor(G-CSF) and granulocyte macrophage-colony stimulating factor(GM-CSF) are principal cytokines in granulopoiesis and their physiologic effects are mediated through binding to specific cell surface receptors. Although it is known that the level of serum G-CSF and GM-CSF, and presentation of the receptors are increased in infectious diseases, there have been no studies to find the correlation between the granulopoiesis and leukocytosis. This study was designed to measure G-CSF and GM-CSF in leukocytosis and in control and to demonstrate the possible pathogenesis of granulopoiesis in leukocytosis using quantitative analysis of G- CSF, GM-CSF and their CSFr.METHODS:
The plasma levels of G-CSF, GM-CSF of 13 children without leukocytosis and 14 children with leukocytosis were measured. Counts of cell surface G-CSFr and GM-CSFr were measured by combining anti G-CSFr and anti GM-CSFr monoclonal antibodies to their respective receptors by using quantitative flow cytometric assay.RESULTS:
There was no significant difference betweeen the plasma concentration of G-CSF and GM-CSF in acute leukocytosis and in the control group. However, levels of G-CSFr in acute leukocytosis decreased significantly compared to the control(P=0.012) and the levels of GM-CSFr in both groups revealed no significant difference.CONCLUSION:
Increase in the number of leukocyte in leukocytosis was mediated by increasing the number of neutrophil, and increased plasma concentration of G-CSF may be the cause of neutrophilia. But GM-CSF did not have any influence on leukocytosis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasma
/
Doenças Transmissíveis
/
Citocinas
/
Fator Estimulador de Colônias de Granulócitos
/
Fator Estimulador de Colônias de Granulócitos e Macrófagos
/
Receptores de Fator Estimulador das Colônias de Granulócitos e Macrófagos
/
Receptores de Fator Estimulador de Colônias de Granulócitos
/
Receptores de Superfície Celular
/
Granulócitos
/
Leucócitos
Limite:
Criança
/
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Pediatric Society
Ano de publicação:
2003
Tipo de documento:
Artigo
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