Effects of total saponins of Panax japonicus on human leukemic HL-60 cells / 中西医结合学报
Journal of Integrative Medicine
; (12): 570-2, 2007.
Article
em Zh
| WPRIM
| ID: wpr-449485
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE: To study the effects of total saponins of Panax japonicus (TSPJ) on human leukemic HL-60 cells. METHODS: Human leukemic HL-60 cells were cultured in vitro. The cancer cell vigor was detected by using cell counting kit-8. Nitroblue tetrazolium (NBT) was used for measuring cell reduction. The cell cycle and the expression of differentiation antigen CD11b were detected by flow cytometry. RESULTS: Compared with the negative control group, TSPJ in different concentrations could decrease the vigor of HL-60 cells and the number of cells in S phase and up-regulate the CD11b expression, while the numbers of NBT positive cells and cells in G(0)/G(1) phase in the different concentrations of TSPJ-treated groups were increased. CONCLUSION: TSPJ can inhibit the HL-60 cell growth in vitro. Its mechanism may be related to inhibiting proliferation and inducing cell differentiation and cycle arrest.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Journal of Integrative Medicine
Ano de publicação:
2007
Tipo de documento:
Article