Effect of Benzalkonium, Mitomycin-C and Dexamethasone on Stress in Trabecular Meshwork Cells
Journal of the Korean Ophthalmological Society
;
: 104-108, 2015.
Artigo
em Coreano
| WPRIM
| ID: wpr-45176
ABSTRACT
PURPOSE:
To investigate the effects of benzalkonium chloride (BAC), mitomycin C (MMC) and dexamethasone (DEX) on cellular stress in cultured human trabecular meshwork cell (HTMC) monolayers.METHODS:
HTMCs were cultured in the inner Transwell chamber until confluence and then were exposed to BAC, MMC or DEX for 6 hours. The carboxyfluorescein permeability through the HTMC monolayer was measured using a spectrofluorometer at 532 nm after 2 hours in the outer chamber. The 3-[4, 5 -dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay was used to evaluate cellular viabilities.RESULTS:
The carboxyfluorescein permeability through the HTMC monolayer increased and cell survival decreased with 0.002% BAC (p 0.05).CONCLUSIONS:
BAC and MMC induced cellular toxicity and stress at lower concentrations but did not affect survival of cultured HTMCs.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Permeabilidade
/
Malha Trabecular
/
Compostos de Benzalcônio
/
Dexametasona
/
Sobrevivência Celular
/
Mitomicina
Limite:
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Ophthalmological Society
Ano de publicação:
2015
Tipo de documento:
Artigo
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