Study on quality standard of Tongfu navel plaster / 国际中医中药杂志

ABSTRACT

Objective To establish the quality standard of Tongfu navel plaster. Methods Rhubarb, Magnolia and Citrus aurantium were identified by thin-layer chromatography(TLC). HPLC method was established for the determination of emodin and rhein. The chromatographic column was SunFire C18 column (4.6 mm×250 mm, 5 μm) with methanol-0.1%H3PO4(85∶15) as the mobile phase. The flow rate was 1.0 ml/min, column temperature was at 30 ℃, and the detection wavelength was 254 nm. Results Within the range of 0.144-1.728μg, emodin presented a fine linear relationship, r=0.999 6. Within the range of 0.016-0.208μg, rhein presented a fine linear relationship, r=0.999 6. The recovery of rhein and emodin was 98.18%and 98.75% with RSD 0.98% and 0.91% respectively. Conclusion The methods are reliable, accurate, reproducible, and can be used for the quality control of Tongfu navel plaste.