Study of bone marrow srt omal cells on a three-dimensional nano-zirconia porous scaffold for bone tissue engineering / 医学研究生学报

ABSTRACT

Objective Nowadays, in the field of bone tissue engineering , searching for a kind of scaffold , which is both strong enough and biocompatible , is still a hot topic .Objective of this study is to evaluate the three-dimensional nano-zirconia porous scaffold′s effect on bone marrow stromal cells early attachment and proliferation . Methods Preparing nano-zirconia porous scaffold and testing its porosity and its mechanical strength .Hemolysis experiment was taken to evaluate its biosafety .Canine bone marrow stromal cells ( BM-SCs) were extracted from an adult beagle and cultured in vitro.The third generation cells were seeded onto the scaffold and induced to os-teoblasts under condition medium .Cells early attachment on scaffold was observed by SEM .MTT and ALP tests were taken to evaluate cells proliferation and osteogenesis within two weeks . Results Nano-zirconia porous scaffold in our study presented a porous structure with a well connected inner .Its porosity and compressive strength were (92.667 ±0.324)%and 4.38 MPa, respectively.Hemolysis rate was 2.19%, which was demonstrated to be safe to human body .Canine BMSCs could colonize the surface of the materials , bridge macro-poros-ity and proliferate on the scaffold .A values taken by the method of MTT showed that cells could proliferate quickly on the scaffold after 3, 6, 9, 12 d (A values were 0.604 ±0.059, 1.215 ±0.019, 1.385 ± 0.254, and 1.506 ±0.050), comparing with that on the first day (A value was 0.261 ±0.034).Statistical difference was found (P <0.01) .The ALP level raised up significantly on day 7 and 14 ( A val-ues were 0.032 ±0.002 and 0.062 ±0.007), comparing with that on the first day (A value was 0.016 ±0.003).Statistical difference was found (P<0.01). Conclusion The three-dimensional nano-ZrO2 porous scaffold in our study presented satisfactory biocompati-bility.It could offer a microenvironment for osteoblasts growth and secreted osteogenesis marker in vitro .