Effects of siRNA targeting livin gene on proliferation and apoptosis of nasopharyngeal carcinoma cell line CNE-2Z / 中国免疫学杂志

ABSTRACT

To observe the effect of livin gene-specific siRNA interference on proliferation and apoptosis of nasopharyngeal carcinoma (NPC) cell line CNE-2Z.Methods: siRNA expression vectors pGPU6/GFP/Neo-livin were transfected into NPC cell line CNE-2Z by using Lipofectamine 2000.The expressions of livin mRNA and protein were detected by semi-quantitative reverse transcriptase-polymerase chain reaction ( RT-PCR) and Western blot.The changes of caspase-3 activity were assessed by kinase semi-quantitative activity test.The proliferation activity and apoptosis of CNE-2Z cells were examined by MTT and flow cytometry respectively.Results:The expression levels of livin mRNA and protein in pGPU 6/GPF/Neo-livin transfected CNE-2Z cells were significantly lower than those in untreated and pGPU 6/GFP/Neo-shNC ( control non-target siRNA ) transfected cells with the expression inhibitory rate of 64.38% and 61.43% respectively.The caspase-3 activity and the apoptotic rate of experimental group cells were increased obviously.The growth of CNE-2Z cells was inhibited by siRNA recombinant expression vector transfection.Conclusion:siRNA targeting livin gene inhibits proliferation and induce apoptosis of CNE-2Z cells.