Establishment of platelet antigen panel cells and application in platelet alloantibody detection / 重庆医学

ABSTRACT

Objective To establish the platelet antigen panel cells and to apply them in the detection and identification of platelet alloantibody.Methods Human platelet antigen(HPA)1-16 genotyping from 1 500 un-related blood donors in Nanning area were performed by the polymerase chain reaction-sequence specific primers(PCR-SSP)technique,platelet antigen cells with O blood type were chosen to establish the panel cells of platelet antigen.The phenotype of the panel cells were verified by the reference sera from the 14th platelet immunology workshop of the International Society of Blood Transfusion(ISBT).And then these panel cells were used in clinic to detect the platelet alloantibody and the samples from the 14th and 15th platelet immunology workshop of ISBT.Re-sults Six platelet cells with consistent phenotype and genotypes and covering the HPA 1-5 and 1 5 systems were selected to estab-lish the platelet panel cells and successfully applied them in the clinical and scientific sample detection and identification.Conclusion Platelet antigen panel cells are established successfully,which provides the experimental basis for the diagnosis and research of platelet allogenic abnormal immunity diseases.