Expression of the receptor for advanced glycation end products in mice with chronic adriamycin-induced cardiotoxicity / 解剖学报

ABSTRACT

Objective To study the expression of receptor for advanced glycation end products ( RAGE ) in chronic adriamycin ( ADR )-induced cardiotoxicity mice . Method A mouse model of chronic cardiotoxicity was established by intraperitoneal injection of 5 mg/kg ADR once a week for continuous 3 weeks.Mice treated with the same volume of saline were used as control group (6 mice in each group).Echocardiography and histopathological examination were used to evaluate the myocardial injury , cardiac function and ventricular remodeling .The expression of RAGE in cardiac tissues was detected by immunohistochemical staining and Western blotting .Results Echocardiography indicated that ADR-treated mice had reduced left ventricular ejection fraction , while histopathological analysis of cardiac tissues showed obvious cardiac damage . RAGE protein in cardiac tissues was highly expressed in chronic ADR-induced cardiotoxicity mice .Conclusion RAGE is highly induced and may be involved in the cardiotoxicity after ADR treatment .