Preliminary application of nucleic acid detection technique in detection of unpaid blood donors in Wuxi region / 国际检验医学杂志

ABSTRACT

Objective To understand the residual risk of transfusion blood donation in the native region and to conduct the sim-ple comparison of Roche diagnostic company′s first generation and second generation nucleic acid combined detection reagents . Methods The menstrual blood specimens qualified by the routine test in this center (serology test and enzyme immunodetection as-say results were negative) from May to July 2013 were selected and performed the 6-specimen mixed sample test by adopting the Roche Diagnostics company′s first-generation and second-generation nucleic acid combined detection reagents in the United Roche COBAS s 201 operating system ,if the test had the reactivity ,the nucleic acid identification tests and other complementary serologi-cal testing were performed .Results 9 417 specimens were detected ,8 cases of nucleic acid positive were screened out with the posi-tive rate of 0 .85 ‰ ,through the detection of nucleic acid identification reagents ,4 specimens were confirmed as positive for HBV nucleic acid ,1 case was positive for HCV nucleic acids and the rest 3 cases of uncertain HBV-positive .In addition ,with the first generation reagent (reference reagents) as the relative standard ,the second generation reagents (assessment reagent) had the over-all positive coincidence rate of 66 .67% ,the negative coincidence rate of 99 .98% and the total coincidence rate of 99 .96% .In addi-tion ,no HIV nucleic acid positive specimens was found by the nucleic acid test .Conclusion The nucleic acid detection technique can effectively shorten window period,further improve the safety of blood transfusions ,at the same time the overall conformance situ-ation of the Roche′s first-generation nucleic acid combined detection reagents and the second generation combined detection reagents is good ,but there are still lesser differences in the detection of HBV .