DNA sequencing of HEV ORF3 cDNA and expression in HepG2 cell / 重庆医学
Chongqing Medicine
;
(36): 3034-3036, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-455961
ABSTRACT
Objective To analysis the DNA sequence of HEV ORF3 cDNA and express in HepG2 cell .Methods Recombinant vector carrying HEV ORF3 cDNA was constructed and identified by DNA sequencing .Expression of recombinant vector in HepG2 cell was determined by immunofluorescence technique .Results The recombinant vector was constructed and expressed in HepG2 cell successfully .Conclusion The research laid experimental foundation for exploring the biological function of ORF3 protein in the pathogenesis of viral hepatitis E .
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chongqing Medicine
Ano de publicação:
2014
Tipo de documento:
Artigo
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