Research on Mesenchymal Stem Cells Differentiation by Serum from Hepatic Fibrosis Rats by Acupuncture on Acupoint and Connective Tissues in Vitro / 世界科学技术-中医药现代化

ABSTRACT

This study was aimed to discuss cellular mechanism of anti-liver fibrosis by acupuncture on acupoint and connective tissues. Rats were divided into five groups, which were the normal group, liver fibrosis model group (12 week), liver fibrosis model group(20 week), acupoint acupuncture group, and connective tissue acupuncture group. The observation was made on morphology of liver in various groups by HE stain and Mallory stain. Concentrations of TG, HA, LN and PC-Ⅲin serum were also detected. Mesenchymal stem cells (MSCs) were intervened by serum from each group to observe MSCs proliferation and differentiation. The results showed that the serum level of model group(12 week) was significantly different from other groups. And the same results were found in the inspection of morphology. In the normal group there were no cell expressing albumin. In other groups, MSCs can express albumin. It was concluded that the serum of normal group cannot make MSCs transform into hepatocyte-like cell. The serum of other groups can make MSCs transform into hepatocyte-like cell. It was concluded that both hepatic fibrosis and acupuncture can induce MSCs differentiation.