Dual-Gradient Liquid Chromatography-Tandem Charged Aerosol Detector for Detection of Macromolecules in Traditional Chinese Medicine Injections / 分析化学

ABSTRACT

A size exclusion chromatography method ( SEC) was established for the screening of macromolecules in traditional Chinese medicine (TCM) injections. In the experiment, TCM was collected through 0. 45μm hybrid filter and separated by SEC with MAbPac SEC-1 column. Acetonitrile was added by post-column mode with a flow rate of 0. 2 mL/min to enhance the detection sensitivity, and 25 mmol/L ammonium formate was used as the mobile phase at flow rate of 0. 2 mL/min. The charged aerosol detection was carried out with Dextran and Tween as the reference standards. The molecular weight distribution of Dextran ranged from 2500 to 133800 had a good linear relationship with the retention time. No macromolecules were detected out in the selected traditional Chinese medicine injections except Kuhuang injections.