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Determination of Puerarin, Daidzin and Daidzein in Puerariae Lobatae Radix by ILDLPME Coupled with HPLC / 中国中医药信息杂志
Chinese Journal of Information on Traditional Chinese Medicine ; (12): 67-70, 2014.
Artigo em Chinês | WPRIM | ID: wpr-459092
ABSTRACT
Objective To develop a sensitive and specific HPLC method to simultaneously determine the contents of puerarin, daidzin and daidzein in Puerariae Lobatae Radix. Methods The three compounds were obtained by ionic liquid based on dispersive liquid phase microextraction. The determination was carried out on a Phenomenex C18 column (250 mm×4.6 mm, 5μm) with a mixture of methanol-0.2%acetic acid (volume ration 45∶55) as mobile phase at a flow rate of 0.8 mL/min. The UV detective wavelength was 250 nm, and the column temperature was set at 35 ℃. Results The linear response ranged from 6.24×10-6-37.44 μg for puerarin (r=0.999 71), 5.44×10-6-27.20μg for daidzin (r=0.999 85), and 5.60×10-6-28.00μg for daidzein (r=0.999 94), respectively. Conclusion The method is quick, simple and repeatable for simultaneous determination of the contents of puerarin, daidzin and daidzein in Puerariae Lobatae Radix.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Journal of Information on Traditional Chinese Medicine Ano de publicação: 2014 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Journal of Information on Traditional Chinese Medicine Ano de publicação: 2014 Tipo de documento: Artigo