Content Determination Method of α-asarone in Dianxianqing Granules / 中国中医药信息杂志
Chinese Journal of Information on Traditional Chinese Medicine
; (12): 83-85, 2014.
Article
em Zh
| WPRIM
| ID: wpr-459143
Biblioteca responsável:
WPRO
ABSTRACT
Objective To discuss the content determination method ofα-asarone in Dianxianqing Granules. Methods Kromasil C18 column (250 mm×4.6 mm, 5 μm) was used, with methanol and water as mobile phase of gradient elution. The flow rate was 1.0 mL/min, and the detection wavelength was 257 nm. The column temperature was 25 ℃. Results The sample solution was prepared by reflux extracted with 70% ethanol for 20 min. α-asarone had a good linearity in the ranges of 1.24×10-3-7.44×10-3μg (r=0.999 5). The method was quite good in precision, stability and repeatability, and the average recovery ofα-asarone was 98.14%, with corresponding RSD of 2.5%. Conclusion The experiment provides an accurate and fast analytical method for α-asarone determination in compound preparation of traditional Chinese medicine.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Information on Traditional Chinese Medicine
Ano de publicação:
2014
Tipo de documento:
Article