Influence of sample collection and storage on detection of iPTH in patients with hemodialysis / 国际检验医学杂志

ABSTRACT

Objective To evaluate the influence of sample collection and storage on the detection results of intact parathyroid hormone(iPTH).Methods 10 blood samples from the hemodialysis patients were simultaneously injected into four types of blood collection tubepro-coagulation tube,blank tube,EDTA tube and sodium citrate tube.The iPTH levels in the fresh samples within 4 h after blood collection and serum/plasma preserving for 12,24,48 h under 4OC were detected by the Roche cobase411 immunoas-say instrument.Results Serum iPTH in the pro-coagulation tube within 4 h after blood collection was rapidly degraded,while the iPTH level in the blank tube was highest,which was significantly higher than that the serum/plasma preserved for 12,24,48 h.The iPTH level in the EDTA tube and the sodium citrate tube began to degrade only at 48 h.Conclusion It is the first time to reveal that the coagulant has the degrading or inhibiting effect on serum iPTH,which verifies that the EDTA tube is the optimal blood col-lection tube.