Antimicrobial resistance and virulence genes of Shigella sonnei in Chang-sha area / 中国感染控制杂志

ABSTRACT

Objective To investigate the antimicrobial susceptibility and the carrying of virulence genes of Shigel-la sonnei (S.sonnei)in Changsha area.Methods Susceptibilities of 30 S.sonnei strains to 6 kinds of antimicrobial agents (ampicillin,cefotaxime,nalidixic acid,ciprofloxacin,gentamicin,compound sulfamethoxazole)were detec-ted by disc diffusion method;4 virulence genes (set1 ,sen,ial,ipaH)were amplified by polymerase chain reaction (PCR).Results Of all detected antimicrobial agents,ampicillin,nalidixic acid and compound sulfamethoxazole had the highest resistance rate(100%);while ciprofloxacin and gentamicin had higher susceptibility rate (93.33% and 83.33% respectively).PCR revealed that the carrying rate of set1,sen,ial and ipaH in 30 S.sonnei strains was 0, 13.33%,13.33% and 53.33% respectively.Conclusion Resistance of S.sonnei to the third-generation cefalosporins and sulfonamides are quite serious,third-generation quinolones can still be used as the first choice of drug treatment for S.sonnei infection.Carrying rate of virulence genes are low among S.sonnei in Changsha area.