Identification of novel HLA alleles：HLA-A*24:233 and HLA-A*26:89 / 中国组织工程研究

ABSTRACT

BACKGROUND:The discovery of novel HLA aleles is accelerated by development of molecular biology and applications of numerous new technologies. These new findings not only rich HLA family, but also find a breakthrough point for the study of genetic superiority or disappearance of national gene. OBJECTIVE:To identify two novel HLA-A aleles and to analysis their nucleotide sequences. METHODS:The two samples from two volunteers of Chinese Marrow Donor Program were detected using PCR-SBT and GSSP methods. The HLA-A locus in the two samples were both abnormal genes, and the nucleotide sequence differences were analyzed. RESULTS AND CONCLUSION: The sequences of the samples were different from al aleles in the HLA databases. Sample 1 was found to be different from the closet matching alele A*240201 in one nucleotide substitutions, 360 G > C in exon 3, resulting in amino acid changed from glutamine to histidine at codon 96; and sample 2 differed from A*260101 in one nucleotide substitution, 97 T > C in exon 2, resulting in amino acid changed from tyrosine to histidine at codon 9. The novel aleles were identified and assigned the name HLA-A*24233 and HLA-A*2689 officialy by the WHO Nomenclature Committee.