Quality Evaluation of Yinhuang Tablets via Digitized and Quantified Fingerprints by High Performance Liquid Chromatography and Seven Components Detection / 医药导报

ABSTRACT

Objective To establish the quality control of Yinhuang tablet (YHT) by using sever ingredients determina-tion and tri-wavelength fingerprint analysis technologies. Methods The chromatographic fingerprints were developed by using Agilent Poroshell 120 SB C18(4. 6 mm×150 mm, 2. 7 μm) as the separation column and 0. 2% H3 PO4-methanol as the mobile phase. The flow rate was 0. 5 mL·min-1 , the UV absorbance was monitored at 214, 278 and 326 nm by injecting 5 μL of the sample solution and the column temperature was maintained at (35. 00±0. 15) ℃ . The chromatographic fingerprints were charac-teristically evaluated for quality ranking of 13 batches of YHT by the 4. 0 software of the digitized evaluation system of traditional chinese medicine fingerprint with the super information characteristics. Seven marker compounds including chlorogenic acid, caf-feic acid, baicalin, wogonoside, baicalein, wogonin and scutellarin were simultaneously determined to compare the variation a-mong five different manufacturers. Results The tri-wavelength fingerprint high performance liquid chromatography method was established. A total of 28(214 nm),29(278 nm)and 26(326 nm)common peaks were identified at respective wavelengths, using baicalin (BCL) as the referential peak. The software was applied to evaluate the YHT-HPLC fingerprints and identify the quality of 13 batches of YHT by systematically quantified fingerprint method (SQFM). The results showed that the content from the dif-ferent manufacturers varied greatly. Conclusion The quality of YHT varies between different makers, and is necessary to es-tablish the criterion for quality control. This method can provide a new reference for the quality control of YHT.